| Timothy Powell | 2-Jun-97 | Lab Section #4 | ||
| Unknown #1 | Pseudomonas fluorescens | |||
| Environmental effects: | ||||
| Test | Unknown | Comments | ||
| Growth at 4 C | 0 | no growth | ||
| Growth at 20 C | 2 out of 5 | slight growth | ||
| Growth at 35 C | 3 out of 5 | mod. growth | ||
| Growth at 45 C | 0 | no growth | ||
| Growth at 55 C | 0 | no growth | ||
| Anaerobic growth | 0 | no growth | ||
| Colony characteristics: | ||||
| Observation | Unknown | |||
| Form? | Circular | |||
| Elevation? | Raised | |||
| Margin? | Entire | |||
| Consistency? | Effuse | |||
| Pigmentation? | ||||
| (1) pigment color? | Cream colored | |||
| Colony diameter, mm | 1.5mm | |||
| Microscopic characteristics: | ||||
| Test | Unknown | Comments | Positive Control | Negative Control |
| Gram stain? | Negative | Staphylococcus epidermis | Escherichia Coli | |
| Acid-fast stain? | Negative | Mycobacterium phlei | Bacillus thuringiensis | |
| Cell morphology? | Bacillus | Elongated rod | Bacillus thuringiensis | Micrococcus luteus |
| Cell size, um? | 5-7um | |||
| Cell arrangement? | Isolated cells | |||
| Capsules | Positive | Capsule present | Klebsiella pneumoniae (capsule) | Micrococcus luteus (no capsule) |
| Endospores? | Negative | All pink | Bacillus thuringiensis | Staphylococcus epidermis |
| "Biochemical" (Metabolic) Test Reactions: | ||||
| Test | Unknown | Comments | Positive Control | Negative Control |
| Glucose fermentation | Negative | Pellicle, sediment | ||
| Lactose fermentation | Negative | Sediment | ||
| Maltose fermentation | Negative | Pellicle, sediment | ||
| Mannitol fermentation | Negative | Sediment | ||
| Sucrose fermentation | Negative | Sediment | ||
| Methyl Red test | Negative | Sediment | Escherichia Coli (red) | Enterobacter aerogenes (yellow) |
| Voges-Proskauer test | Negative | Sediment | Enterobacter aerogenes (black line) | Escherichia Coli (plain) |
| Catalase test | Positive | Bubbles | Pseudomonas | Streptococcus faecalis |
| Oxidase test | Positive | Medium grey | Pseudomonas | Streptococcus faecalis |
| Starch hydrolysis | Negative | Plate gouged | Bacillus subtilis (clearing) | Escherichia Coli (no clearing) |
| Casein hydrolysis | Negative | Moderate growth | Bacillus subtilis (ate milk) | Escherichia Coli (didn't eat milk) |
| Gelatin hydrolysis | Negative | Clear | Bacillus subtilis (liquefied) | Escherichia Coli (gel) |
| Indole production | Negative | Pellicle | Escherichia Coli (red) | Enterobacter aerogenes (clear) |
| H2S Production | Negative | Pellicle | Proteus vulgaris (black) | Enterobacter aerogenes (clear) |
| Lysine decarboxylation | Negative | Sediment | Enterobacter aerogenes (purple) | Citrobacter freundi (yellow) |
| Phenylalanine deaminase | Negative | Proteus vulgaris (green) | Escherichia Coli (yellow) | |
| Citrate utilization | Positive | Enterobacter aerogenes (blue) | Citrobacter freundi (green) | |
| Ammonification | Positive | Dark yellow | 4% Peptene broth | |
| Nitrate reduction | Positive | Gas and bubbles | Soil (gas) | |
| Urea hydrolysis | Negative | Sediment from tablet | ||