Discussion

In the lab experiment performed the DNA (a long linear polymer, composed of four kinds of deoxyribose nucleotides, linked by phosphodiester bonds, that is the carrier of genetic information -Lodish: G6) of an onion was isolated and extracted from the rest of the cell tissue. Upon visual inspection the results proved favorable and a visually significant piece of DNA was extracted. From this, a conclusion can be made that the isolation and extraction procedures outlined in the lab experiment can and do work. However, further analysis of the extracted DNA by light microscopy will be needed to determine the full success of the extraction procedure. Perhaps by running a series of extraction's using onions and related plant species the viability of the extraction procedure can be more fully assessed.

As part of the lab experiment another onion DNA extraction was performed without using cold absolute ethanol. The results were that no alcohol layer was formed and no precipitate could be observed or collected. From this, a conclusion can be reached that the ethanol must react with the onion DNA either on a mechanical or chemical basis and exert a force necessary to extract the DNA from the rest of the cellular components. The failure to extract and isolate the onion DNA however does not mean that DNA was not present in the interface layer of the centrifuge tube. In order to test whether the ethanol plays a critical role in the extraction, a series of experiments could be done using other alcohol's similar in structural formula to ethanol. A thorough understanding of Organic Chemistry would be needed to facilitate this.

References

Lodish; Molecular Cell Biology, Third Edition. Scientific American Books: New York, " 1995.