In the lab experiment performed the DNA (a long linear polymer,
composed of four kinds of deoxyribose nucleotides, linked by phosphodiester
bonds, that is the carrier of genetic information -Lodish: G6)
of an onion was isolated and extracted from the rest of the cell
tissue. Upon visual inspection the results proved favorable and
a visually significant piece of DNA was extracted. From this,
a conclusion can be made that the isolation and extraction procedures
outlined in the lab experiment can and do work. However, further
analysis of the extracted DNA by light microscopy will be needed
to determine the full success of the extraction procedure. Perhaps
by running a series of extraction's using onions and related plant
species the viability of the extraction procedure can be more
fully assessed.
As part of the lab experiment another onion DNA extraction
was performed without using cold absolute ethanol. The results
were that no alcohol layer was formed and no precipitate could
be observed or collected. From this, a conclusion can be reached
that the ethanol must react with the onion DNA either on a mechanical
or chemical basis and exert a force necessary to extract the DNA
from the rest of the cellular components. The failure to extract
and isolate the onion DNA however does not mean that DNA was not
present in the interface layer of the centrifuge tube. In order
to test whether the ethanol plays a critical role in the extraction,
a series of experiments could be done using other alcohol's similar
in structural formula to ethanol. A thorough understanding of
Organic Chemistry would be needed to facilitate this.
Lodish; Molecular Cell Biology, Third Edition. Scientific American Books: New York, " 1995.